Optical Filers by Application
Versatile in Ise and Crucial for Measurements
The application possibilities of high-quality and durable optical filters are manifold. Classically, they are used for measuring methods with microscope in the field of basic research in biology, physics, chemistry, medicine, pharmacy, food, material sciences, materials engineering etc. or contract research and for professional testing laboratories. In addition, they are also used in industrial research and development or for quality testing in the production process. Interesting new fields of application are increasingly emerging in automated industrial image processing (machine vision) and artificial intelligence (AI), for example, when it comes to filtering out unwanted or unneeded image information. Please find here some examples of applications in the field of microscopy and spectroscopy:
(Image of FRET, Multiphoton and TIRF provided by » IDEX | Semrock)
Raman Spectroscopy
Raman spectroscopy, similar to IR spectroscopy, measures vibrational and rotational transitions of molecules. The difference is that Raman spectroscopy does not use IR light, but visible light.
TIRF Microscopy
Within wide-field microscopy, Total Internal Reflection Fluorescence (TIRF) microscopy has emerged as a routine method by which significant contrast enhancement of the sample can be achieved in the evanescent field. To achieve the TIRF mode, attention must be paid to the appropriate filter specification, among other things.
PCR Diagnostics
PCR diagnostics enables DNA to be detected and sequenced. RT-PCR is used to detect or sequence RNA. In a first step, the RNA is translated into DNA with the help of reverse transkritase and then amplified in the polymerase chain reaction (PCR).
Multiphoton Microscopy
In multiphoton microscopy, molecules are transferred to an electronically excited state by several photons. From this state, these molecules relax back to the electronic ground state by emitting a photon.
FRET Microscopy
Förster or fluorescence resonance energy transfer (FRET) microscopy is a special application of fluorescence microscopy. In the FRET effect, energy is transferred from a donor molecule to an acceptor molecule. Due to the strong distance dependence of the effect, distances can be measured very accurately.
Reader & FACS
Flow cytometry or fluorescence-activated cell sorting (FACS) is a key technology that enables the analysis of cells down to subcellular structures in suspension. The aim is to identify structural and functional properties of e.g. blood cells.
FISH
In fluorescence in situ hybridization (FISH), nucleic acids (DNA or RNA) can be detected in cells by using an artificial probe made of nucleic acid and the simultaneous use of different fluorescent dyes.
Do You Need a Special Optical Filter for Your Application?
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