A Perfect Match for DNA Diagnostics with FISH
Microscope- and Dye-specific Optical Filters and LED Light Sources
Fluorescence in situ hybridization (FISH, mFISH) is a common method to approach cytogenetic analysis of nucleic acids. Precise selection of optical filters and light sources plays a key role to achieve brilliant fluorescence signals with almost no background and cross talk. AHF analysentechnik offers filter sets consisting of excitation filter, emission filter and beamsplitters as well as state-of-the-art LED light sources with optimized spectrum for FISH applications. Each filter of the set will be carefully chosen to match both the specific microscope type and fluorescence dyes used. Demo filter sets and light sources can be provided for testing purposes in your lab facility. AHF is happy to give you support in selection of the right filter and light source for your specific application.
LED Light Sources for FISH: The Better Way of Illumination
It is recommended to use LED illumination for fluorescence excitation as it provides many benefits to the FISH application user. LED light sources are completely mercury-free and have a 10–100 times longer lifetime (up to 35,000 hours) compared to arc lamps. There is no need for re-calibration after lamp exchange due to no lamp replacement.
Additionally, they produce much less radiation/heat and can be switched on/off instantaneously as no warm-up or cooling-down time has to be considered. In a medium term, LED light sources reduce the cost of operation and maintenance. AHF offers the newest generation of LED light source for FISH applications like Lumencor’s SOLA FISH or the new AF-2100.
For Power Users: Lumencor SOLA FISH
- Highest output power avg. > 5000 mW
- Spectral output 365 – 680 nm
- Lifetime > 20,000 hours
- Control via software/controller
- Output in the 475–600 nm region is red-shifted to provide optimal excitation for fluorophores commonly used for FISH analysis
For Daily Routine: AF-2100 FISH
- High output power avg > 4000 mW
- Spectral output 365 – 680 nm
- Silent and vibration-free
- Direct microscope coupling
- Lifetime > 25,000 hours
- Control via software/controller
FISH Filter Sets: Dye and Microscope-specific
AHF’s filter portfolio compromises specific wavelengths of fluorescence probes from Abbott, MetaSystems, ZytoVision, Cytocell, Kreatech (Leica) etc. Also available are multiband filter sets with two or three transmission bands that allow to detect up to three different dyes simultaneously. All sets will be provided completely mounted in a microscope filter cube.
Optical Singleband Filter Sets
- Series of specific narrowband mFISH filter sets
- High transmission, minimum crosstalk
- For kits stained with
DAPI – Blue – Aqua – Green – Gold – Orange – Red – Far Red – NIR – IR - Matched to probes from Abbott, MetaSystems, ZytoVision, Cytocell, Kreatech (Leica)
Optical Dualband Filter Sets for mFISH
- Excellent for routine analysis
- Quick screening of Green/Orange-Red signals and Yellow colocalization signals
- Various multiband sets are available, fitting to various microscope set-ups