Maximize Fluorescence with the New Avant™ Filter Set Family
While every fluorescent filter set has a Gap separating excitation and emission passbands, many sets for short Stokes Shift probes have large Gaps that shift the emission filter to longer wavelengths. The emission filter captures less fluorescence and the filter set’s performance is therefore reduced. Limitations in the coating process of the filters resulted in signal acquisition not being optimal in some cases for the filter sets available so far. IDEX Health & Science’s new Semrock Avant™ technology narrows this Gap and brings the emission and excitation passbands close together. The Avant™ family of sets thereby deliver significant increases in fluorescence signal.
The secret? Steep spectral edges and high OD blocking of the excitation passband in the emission filter. The spectral features are confirmed by Semrock’s high-resolution KolaDeep™ Spectral Measurement System, and the new Precise Edge Placement capability assures that every filter set delivers optimum performance.
Application areas for the Avant™ filter series are:
- Fluorescence microscopy
- Next Generation Sequencing
- High Throughput/Content Screening
- Flow cell and microarray ('Biochip') Readers
- Real-time PCR
- Flow cytometry
- Chemical process monitoring
- And many more